Regulation of the Cyanobacterial Bidirectional Hydrogenase
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منابع مشابه
Transcriptional regulation of the cyanobacterial bidirectional Hox-hydrogenase.
The overall processes of transcription and its regulation have advanced significantly in the last years, making our understanding of prokaryotic biology more complex and detailed. In fact, a systematic study of different aspects of transcriptional regulation opens up outstanding opportunities to improve and develop the perception of complex reaction mechanisms, genetic processes and cell functi...
متن کاملInhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803
BACKGROUND Molecular hydrogen is an environmentally-clean fuel and the reversible (bi-directional) hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 as well as the native Escherichia coli hydrogenase 3 hold great promise for hydrogen generation. These enzymes perform the simple reaction 2H+ + 2e- <--> H2 (g). RESULTS Hydrogen yields were enhanced up to 41-fold by cloning the bidire...
متن کامل[NiFe]-hydrogenase is essential for cyanobacterium Synechocystis sp. PCC 6803 aerobic growth in the dark
The cyanobacterium Synechocystis sp. PCC 6803 has a bidirectional [NiFe]-hydrogenase (Hox hydrogenase) which reversibly reduces protons to H2. This enzyme is composed of a hydrogenase domain and a diaphorase moiety, which is distinctly homologous to the NADH input module of mitochondrial respiratory Complex I. Hox hydrogenase physiological function is still unclear, since it is not required for...
متن کاملHydrogenases and hydrogen metabolism of cyanobacteria.
Cyanobacteria may possess several enzymes that are directly involved in dihydrogen metabolism: nitrogenase(s) catalyzing the production of hydrogen concomitantly with the reduction of dinitrogen to ammonia, an uptake hydrogenase (encoded by hupSL) catalyzing the consumption of hydrogen produced by the nitrogenase, and a bidirectional hydrogenase (encoded by hoxFUYH) which has the capacity to bo...
متن کاملII.G.3 Hydrogen from Water in a Novel Recombinant Oxygen-Tolerant Cyanobacterial System
Re-engineered transcriptional regulation of hydrogenase • to achieve four-fold higher activity (from 0.5 to 2 nmol H2*mg protein *h). Examined the effect of transcriptional modifications on • hydrogenase maturation and found that proper regulation of accessory genes is essential for optimal hydrogenase maturation. Created mutants of the environmentally-derived • hydrogenase small subunit with f...
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تاریخ انتشار 2008